Poster Presentation 29th Lorne Cancer Conference 2017

Inflammation mediates profound changes to the immunopeptidome of melanoma (#290)

Katherine Woods 1 2 , Ralf B Schittenhelm 3 , Anthony W Purcell 3 , Andreas Behren 1 2 , Jonathan Cebon 1 2
  1. School of Cancer Medicine, La Trobe University, Melbourne, VIC, Australia
  2. Cancer Immunobiology, Olivia Newton-John Cancer Research Institute, Melbourne, VIC, Australia
  3. Biochemistry and Molecular Biology, Monash University, Melbourne, VIC

Degradation of cellular proteins by the proteasome is critical for generation of MHC-associated peptides. The constitutive proteasome and the IFNγ-induced immunoproteasome (IP) differ in the use of three catalytic β subunits, which alters production of MHC class I epitopes. The potential for a disparate repertoire of epitopes between inflammatory/non-inflammatory tumours therefore arises. We have previously demonstrated this phenomenon as a mechanism of escape from T lymphocyte killing following changed processing of the cancer-testis antigen NY-ESO-1 [1].

Based on our, and others, prior data demonstrating alterations in the epitopes produced following processing of individual antigens, it follows that whole scale plasticity of the immunopeptidome could arise depending on which proteasome subtype is expressed by a tumour.  Thus, the potential arises for profound change in the ability of CD8+ T lymphocytes to recognise tumor cells, depending on IFNɣ mediated inflammation. Given the change in targetability of tumour cells we observed following changed processing of a single antigen, a wholescale alteration of the immunopeptidome may represent a significant mechanism of immune escape by the tumour. Such plasticity may occur temporally or heterogeneously within a single tumour. 

Here, we present the first study demonstrating the effect of IFNɣ mediated inflammation on the composition of the immunopeptidome in melanoma. Using a sensitive mass spectrometry approach (DIA-SWATH) we characterise changes to the immunopeptidome of melanoma, dependent on the proteasome subtype expressed by the cell. These changes dramatically altered the landscape of the cell. Significantly, we demonstrate that while the immunopeptidome had similar overall immunogenicity in presence or absence of IFNɣ, there were changes in the ability of CD8+ T cells to recognise altered epitopes. 

Importantly, this study highlights the effect of changes in inflammation at the tumour microenvironment as a potential mechanism of significant immune escape from T lymphocyte mediated tumour targeting. Awareness of tumour immunopeptidome plasticity may be critical to inform development of therapies involving cancer vaccination, adoptive T-lymphocyte transfer, or combination treatments including these.

  1. (1) Woods, K., Knights, A.J., Anaka, M., Schittenhelm, R.B., Purcell, A.W., Behren, A., and Cebon, J. (2016). Mismatch in epitope specificities between IFNgamma inflamed and uninflamed conditions leads to escape from T lymphocyte killing in melanoma. J Immunother Cancer 4, 10.